Abstract
The contents of neomycin in a gíven pharmaceutical product can be promptly verified through the reaction of neomycin with ninhidryn in glycerinic medium and under certain pre-established conditions. The method herein described is based on the reaction of neomycín with the ninhidryn reagent containing glycerin. Under such conditions the reaction becomes more perceptible and its results reproducible. Beer law applíes to this case in a range between 4 to 40 flg neomycin sulfate per ml. Some interfering agents were studied as well. In cases where the neomycin was to be separated prior to the reaction, a thín-layer chromatography method was used.
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