Determination of the soluble dietary fiber fraction in yacon (Smallanthus sonchifolius) root and flour by enzymatic-gravimetric method and high pressure liquid chromatography
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Keywords

dietary fiber
yacon root
yacon flour
inulin
frutoligosaccharides

How to Cite

1.
Vasconcelos CM, Silva CO da, Teixeira LJQ, Chaves JBP, Martino HSD. Determination of the soluble dietary fiber fraction in yacon (Smallanthus sonchifolius) root and flour by enzymatic-gravimetric method and high pressure liquid chromatography. Rev Inst Adolfo Lutz [Internet]. 2010 Feb. 1 [cited 2024 May 18];69(2):188-93. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32655

Abstract

This study aimed at determining the total dietary fiber contents in yacon root pulp (YRP) and yacon flour (YF) by the AOAC enzymatic-gravimetric method, and fructans by high performance liquid chromatography (HPLC). The YF was put through the process of cleaning, sanitizing, bleaching, and YRP was dried at 55 °C for 48 hours. The chemical composition of the YF and YRP was determined, being the total fiber contents
the sum of the determinations by enzymatic-gravimetric method and HPLC. High contents of total dietary fiber were detected in the dry mater of both YRP and YF samples (33.14 and 50.76%, respectively), being mostly fructooligosaccharides [FOS] (37.97 and 40.97%, respectively), and substantial inulin contents (26.10 and 20.75%, respectively). The chromatogram also showed high contents of fructose, glucose and sucrose as the sole source of carbohydrates besides fructans, inulin and FOS. The enzymatic-gravimetric methods are considered the most suitable for routine analysis of dietary fibers, but are ineffective for determining oligosaccharides with polymerization degree less than 12, as yacon. Thus, a complementary method, such
as HPLC, needs to be carried out to quantify the exacting dietary fiber contents in this root.

https://doi.org/10.53393/rial.2010.v69.32655
PDF (Português (Brasil))

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